AustralieFrV1, Main, Exploration, bibRecord, 00A089

Analysis of the transcript levels of VvAdh1, VvAdh2 and VvGrip4, three genes highly expressed during Vitis vinifera L. berry development

Identifieur interne : 00A089 ( Main/Exploration ); précédent : 00A088; suivant : 00A090

Analysis of the transcript levels of VvAdh1, VvAdh2 and VvGrip4, three genes highly expressed during Vitis vinifera L. berry development

Auteurs : C. Tesniere [France] ; C. Davies [Australie] ; L. Sreekantan [Australie] ; J. Bogs [Australie] ; M. Thomas [Australie] ; L. Torregrosa [France]

Source :

Vitis [ 0042-7500 ] ; 2006.

RBID : Pascal:06-0194917

Descripteurs français

Pascal (Inist)
- Expression génique, Baie(fruit), Fructification, Promoteur, Viticulture, Vitis vinifera, Alcohol dehydrogenase.
Wicri :
- topic : Viticulture.

English descriptors

KwdEn :
- Alcohol dehydrogenase, Berry, Fructification, Gene expression, Promoter, Viticulture, Vitis vinifera.

Abstract

Well defined promoter sequences are required for the targeted expression of genes in transgenic grapevines. This paper describes a detailed study of the expression of three genes with potentially useful promoters. We have used real-time RT-PCR to evaluate the transcript levels of VvAdh1, VvAdh2 and VvGrip4 genes in various tissues (root, bud, tendril, inflorescence, fruit and embryogenic callus) of Vitis vinifera L. at different stages of development. Transcript levels of the three genes were highest in berry tissues but each had a distinct pattern of expression. VvAdhl showed higher transcript levels during the early stages of berry development, while levels of VvAdh2 and VvGrip4 were higher during ripening. However, none of these genes was expressed in a strictly fruit-specific manner. In particular, significant levels of VvAdhl and VvGrip4 transcripts were observed during late tendril development and in the inflorescence, respectively. Transcript levels of all three genes were similar in both flesh and skin, indicating no tissue-specificity within the berry. Promoter sequences of the VvAdhl, VvAdh2 and VvGrip4 genes could be very useful to drive ectopic gene expression in berries of transgenic plants.

Affiliations:

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Well defined promoter sequences are required for the targeted expression of genes in transgenic grapevines. This paper describes a detailed study of the expression of three genes with potentially useful promoters. We have used real-time RT-PCR to evaluate the transcript levels of VvAdh1, VvAdh2 and VvGrip4 genes in various tissues (root, bud, tendril, inflorescence, fruit and embryogenic callus) of Vitis vinifera L. at different stages of development. Transcript levels of the three genes were highest in berry tissues but each had a distinct pattern of expression. VvAdhl showed higher transcript levels during the early stages of berry development, while levels of VvAdh2 and VvGrip4 were higher during ripening. However, none of these genes was expressed in a strictly fruit-specific manner. In particular, significant levels of VvAdhl and VvGrip4 transcripts were observed during late tendril development and in the inflorescence, respectively. Transcript levels of all three genes were similar in both flesh and skin, indicating no tissue-specificity within the berry. Promoter sequences of the VvAdhl, VvAdh2 and VvGrip4 genes could be very useful to drive ectopic gene expression in berries of transgenic plants.</div>
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Analysis of the transcript levels of VvAdh1, VvAdh2 and VvGrip4, three genes highly expressed during Vitis vinifera L. berry development

Analysis of the transcript levels of VvAdh1, VvAdh2 and VvGrip4, three genes highly expressed during Vitis vinifera L. berry development

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri